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International Journal of Plant Based Pharmaceuticals
e‑ISSN 2791-7509 Open Access · CC BY 4.0
Vol. 3 · Issue 1 · 2023 Jun 15, 2023 Research Articles

Ameliorative effect of lycopene alone and in combination with co-enzyme Q10 in streptozotocin-induced diabetic nephropathy in experimental rats

PR
Pooja B. Rasal SNJB’s Shriman Sureshdada Jain College of Pharmacy, Department of Pharmacology, Chandwad Dist: Nashik, India India
GK
Gaurav N. Kasar SNJB’s Shriman Sureshdada Jain College of Pharmacy, Department of Pharmacology, Chandwad Dist: Nashik, India India
MM
Manoj S. Mahajan SNJB’s Shriman Sureshdada Jain College of Pharmacy, Department of Pharmacology, Chandwad Dist: Nashik, India India
AU
Aman B. Upaganlawar Corresponding SNJB’s Shriman Sureshdada Jain College of Pharmacy, Department of Pharmacology, Chandwad Dist: Nashik, India amanrxy@gmail.com India
CU
Chandrashekhar D. Upasani SNJB’s Shriman Sureshdada Jain College of Pharmacy, Department of Pharmacology, Chandwad Dist: Nashik, India India
Pages123-130 PublishedJun 15, 2023 LicenseOpen Access
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IJPBP 1 VOL 3 · 1
VOL 3 · NO 1 · 2023 View issue

Abstract

Diabetic nephropathy (DN) has become an utmost reason for long-standing renal dysfunction and end-stage renal disease globally. Oxidative stress induced by persistent hyperglycemia is considered a fundamental element in the evolution of DN. The goal of this research was to discover the outcome of appendages of natural antioxidants such as lycopene and co-enzyme Q10 (CoQ10) in DN rats and to observe the preventive effects in DN. A diabetes model was developed in a Wistar strain of male rats (200–250 g) by subcutaneous injection of streptozotocin (55 mg/kg). Development of nephropathy was assessed by renal function tests including blood glucose, creatinine, albumin, total protein, total bilirubin, uric acid, total cholesterol, triglycerides, and CRP level. Oxidative stress markers such as LPO and GSH content and activity of membrane-bound Na + /K + ATPases were measured in kidney homogenate. Renal damage was assessed by performing a histopathological analysis. DN rats showed a significant elevation in creatinine, albumin, total protein, total bilirubin, uric acid, total cholesterol, triglycerides, CRP, and LPO levels whereas a significant reduction in creatinine clearance and GSH level. Treatment with antioxidants such as lycopene (5 mg/kg/p.o.) and CoQ10 (10 mg/kg/p.o.) along with their combination for 4 weeks notably altered the level of renal function biomarkers and oxidative stress markers. These antioxidants and their combination also protected the kidney from abnormal morphological changes. The present findings suggest that the combined administration of lycopene and CoQ10, which are antioxidants, exhibits synergistic effects in mitigating renal injury by reducing hyperglycemia, oxidative stress markers, and histopathological alterations.

Keywords

#Antioxidants #CoQ10 #Diabetic nephropathy #Lycopene #Oxidative stress #Streptozotocin

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Copyright (c) 2023 Pooja B. Rasal, Gaurav N. Kasar, Manoj S. Mahajan, Aman B. Upaganlawar, Chandrashekhar D. Upasani

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