Phytochemical profile and antioxidant potential of extracts of Callicarpa macrophylla Vahl. from Uttarakhand Himalayan region


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Authors

  • Aradhana Arya Govind Ballabh Pant University of Agriculture and Technology, College of Basic Sciences and Humanities, Department of Biochemistry, Pantnagar-263145, Uttarakhand, India https://orcid.org/0000-0003-1142-1575
  • Shiv Kumar Dubey Govind Ballabh Pant University of Agriculture and Technology, College of Basic Sciences and Humanities, Department of Biochemistry, Pantnagar-263145, Uttarakhand, India https://orcid.org/0000-0001-5841-2807
  • Arti Lauthani Govind Ballabh Pant University of Agriculture and Technology, College of Basic Sciences and Humanities, Department of Biochemistry, Pantnagar-263145, Uttarakhand, India https://orcid.org/0000-0001-7201-7037
  • Ananya Bahuguna Govind Ballabh Pant University of Agriculture and Technology, College of Basic Sciences and Humanities, Department of Biochemistry, Pantnagar-263145, Uttarakhand, India https://orcid.org/0000-0003-1142-1575
  • Ravendra Kumar Govind Ballabh Pant University of Agriculture and Technology, College of Basic Sciences and Humanities, Department of Chemistry, Pantnagar-263145, Uttarakhand, India https://orcid.org/0000-0002-0296-5231
  • Om Prakash Govind Ballabh Pant University of Agriculture and Technology, College of Basic Sciences and Humanities, Department of Chemistry, Pantnagar-263145, Uttarakhand, India https://orcid.org/0000-0002-2767-6997
  • Dharmendra Singh Rawat Govind Ballabh Pant University of Agriculture and Technology, College of Basic Sciences and Humanities, Department of Biological Sciences, Pantnagar-263145, Uttarakhand, India https://orcid.org/0000-0003-2613-2709

DOI:

https://doi.org/10.29228/ijpbp.25

Keywords:

Callicarpa macrophylla, DPPH, Reducing power, Antioxidant, Phytochemical

Abstract

The medicinal traits of Callicarpa macrophylla Vahl. have been used in traditional and folklore medicinal system, however, scientific evaluation of such medicinal plants have not been much explored yet. Different solvent extracts of the whole plant of C. macrophylla from the Himalayan region are the key subject of interest to evaluate and exhibit their antioxidant properties concerning its phytochemical composition. The different polarity extracts of C. macrophylla were prepared using the Soxhlet apparatus and both of their GC-MS profile outlined their individual components. The antioxidant activity of both extracts was evaluated for DPPH radical scavenging activity, metal chelating activity, and reducing power activity. Thirty-two compounds were identified in the hexane extract, accounting for 63.56% of the total extract composition with tetratetracontane (9.78%), as the prominent compound. A sum of 26 compounds making up 79.74% of the total methanolic extract composition was identified and the major compound was a diterpenoid, trachylobane (31.87%). The methanolic extract exerted potent antioxidant properties when compared, in vitro, to hexane extract as it showed better IC50 values of DPPH radical scavenging activity (91.97 ± 0.33 µg/ml), metal chelating activity (10.90 ± 1.40 µg/ml) and reducing power activity (56.72 ± 0.67 µg/ml). The antioxidant potential of the methanolic extract was also comparable to standards. Therefore, the methanolic extract exhibited better results as compared to hexane extract and had comparable potential with standards in vitro, if further explored might be utilized as an antioxidant agent in various pharmaceutical and cosmetic industries.

References

Ado, M. A., Abas, F., Leong, S. W., Shaari, K., Ismail, I. S., Ghazali, H. M., & Lajis, N. H. (2016). Chemical constituents and biological activities of Callicarpa maingayi leaves. South African Journal of Botany, 104, 98-104.

Amin, M., Manjang, Y., Ibrahim, S., Efdi, M., & Santoni, A. (2015). Phytochemical screening and antioxidant activity from stem bark Callicarapa arborea Roxb. Journal of Chemical and Pharmaceutical Research, 7(11), 749-755.

Amudha, P., Jayalakshmi, M., Pushpabharathi, N., & Vanitha, V. (2018). Identification of bioactive components in Enhalus acoroides seagrass extract by gas chromatography-mass spectrometry. Asian Journal of Pharmaceutical and Clinical Research, 11(10), 313-315.

Bahuguna, A., Dubey, S. K., Garia, V., Kumar, R., Prakash, O., & Rawat, D. S. (2023). Composition, antioxidant and anti-inflammatory activities of different polarity extracts of Anaphalis busua from the Himalayan terrain of Uttarakhand. European Journal of Chemistry, 14(1), 114-120.

Cai, H., Xie, Z., Liu, G., Sun, X., Peng, G., Lin, B., & Liao, Q. (2014). Isolation, identification and activities of natural antioxidants from Callicarpa kwangtungensis Chun. PLoS One, 9(3), e93000.

Chandra, M., Prakash, O., Kumar, R., Bachheti, R. K., Bhushan, B., Kumar, M., & Pant, A. K. (2017). β-Selinene-rich essential oils from the parts of Callicarpa macrophylla and their antioxidant and pharmacological activities. Medicines, 4(3), 52.

Djeridane, A., Yousfi, M., Nadjemi, B., Boutassouna, D., Stocker, P., & Vidal, N. (2006). Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chemistry, 97(4), 654-660.

Gairola, K., Gururani, S., Kumar, R., Prakash, O., Agarwal, S., & Dubey, S. K. (2021). Phytochemical composition, antioxidant, and anti-inflammatory activities of essential oil of Acmella uliginosa (Sw.) Cass. grown in North India Terai region of Uttarakhand. Trends in Phytochemical Research, 5(1), 44-52.

Gulcin, İ., & Alwasel, S. H. (2022). Metal ions, metal chelators and metal chelating assay as antioxidant method. Processes, 10(1), 132.

Hossain, M. R., Rumi, M. H., Arik, F. H., Khurshed, T., & Chowdhury, A. F. (2019). Pharmacological activity investigation of Callicarpa attenuate. Journal of Pharmacognosy and Phytochemistry, 8(4), 1963-1967.

Junejo, J. A., Rudrapal, M., Nainwal, L. M., & Zaman, K. (2017). Antidiabetic activity of hydro-alcoholic stem bark extract of Callicarpa arborea Roxb. with antioxidant potential in diabetic rats. Biomedicine & Pharmacotherapy, 95, 84-94.

Kala, C. P. (2006). Medicinal plants of the high altitude cold desert in India: diversity, distribution and traditional uses. The International Journal of Biodiversity Science and Management, 2(1), 43-56.

Lam, D. T., Le, V. T. T., Quan, P. M., Minh, P. T. H., Thuy, T. T. T., Anh, N. T. N., Tai, B. H., & Kiem, P. V. (2021). Two new terpenoids from the leaves of Callicarpa macrophylla. Natural Product Research, 35(7), 1107-1114.

Maffei, M. (1994). Discriminant analysis of leaf wax alkanes in the Lamiaceae and four other plant families. Biochemical Systematics and Ecology, 22(7), 711-728.

Mishra, K., Ojha, H., & Chaudhury, N. K. (2012). Estimation of antiradical properties of antioxidants using DPPH assay: A critical review and results. Food Chemistry, 130(4), 1036-1043.

Nawaz, H., Shad, M. A., Rehman, N., Andaleeb, H., & Ullah, N. (2020). Effect of solvent polarity on extraction yield and antioxidant properties of phytochemicals from bean (Phaseolus vulgaris) seeds. Brazilian Journal of Pharmaceutical Sciences, 56, e17129.

Patel, R., & Jawaid, T. (2014). Hepatoprotective activity of aerial parts of plant extract of Callicarpa macrophylla in rats. Pharmacy and Pharmacology Research, 2(1), 1-8.

Patra, K., Jana, S., Mandal, D. P., & Bhattacharjee, S. (2016). Evaluation of the antioxidant activity of extracts and active principles of commonly consumed Indian spices. Journal of Environmental Pathology, Toxicology and Oncology, 35(4), 299-315.

Raja, R. R. (2012). Medicinally potential plants of Labiatae (Lamiaceae) family: an overview. Research Journal of Medicinal Plant, 6(3), 203-213.

Rouzbahan, S., Moein, S., & Homaei, A. (2016). Total antioxidant capacities and reduction of ferric ions by extracts of three medicinal plants and their fractions. Hormozgan Medical Journal, 20(4), 201-209.

Roy, S., Zomuanpuia, J., Laithangliani, R., Hulianthanga, J. L., Hauhnar, L., Lalchhandama, K., Lalhminghlui, K., & Lalthanpuii, P. (2020). Screening of Callicarpa arborea and Hemigraphis alternata for antibacterial activity. Science Vision, 20(2), 72–77.

Samuelsson, G. (2004). Drugs of natural origin: a textbook of pharmacognosy, 5th: Swedish Pharmaceutical Press, Stockholm, Sweden.

Sharma, G. N., Kaur, H., Shrivastava, B., & Arora, S. C. (2021). Comparative in vitro antioxidant activity of ethyl acetate and ethanol extracts of Callicarpa macrophylla. Asian Journal of Pharmaceutical and Clinical Research, 14(5), 31-35.

Shetty, K., Curtis, O. F., Levin, R. E., Witkowsky, R., & Ang, W. (1995). Prevention of vitrification associated with in vitro shoot culture of Oregano (Origanum vulgare) by Pseudomonas spp. Journal of Plant Physiology, 147(3-4), 447-451.

Singh, A. K., Chanotiya, C. S., Yadav, A., & Kalra, A. (2010). Volatiles of Callicarpa macrophylla: a rich source of selinene isomers. Natural Product Communications, 5(2), 269-272.

Singh, G., Passsari, A. K., Leo, V. V., Mishra, V. K., Subbarayan, S., Singh, B. P., Kumar, B., Kumar, S., Gupta, V. K., et al. (2016). Evaluation of phenolic content variability along with antioxidant, antimicrobial, and cytotoxic potential of selected traditional medicinal plants from India. Frontiers in Plant Science, 7, 407.

Zazouli, S., Chigr, M., & Jouaiti, A. (2016). Effect of polar and nonpolar solvent on total phenolic and antioxidant activity of roots extracts of Caralluma europaea. Der Pharma Chemica, 8(11), 191-196.

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Published

14.07.2023

How to Cite

Arya, A., Dubey, S. K., Lauthani, A., Bahuguna, A., Kumar, R., Prakash, O., & Rawat, D. S. (2023). Phytochemical profile and antioxidant potential of extracts of Callicarpa macrophylla Vahl. from Uttarakhand Himalayan region. International Journal of Plant Based Pharmaceuticals, 3(2), 131–140. https://doi.org/10.29228/ijpbp.25

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Section

Research Articles
Received 2023-04-21
Accepted 2023-07-10
Published 2023-07-14