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International Journal of Plant Based Pharmaceuticals
e‑ISSN 2791-7509 Open Access · CC BY 4.0
Vol. 4 · Issue 1 · 2024 Apr 22, 2024 Research Articles

Toxicity study and ameliorative effects of the aqueous leaf extract of Lecanoidiscus cupanioides Planch (ex. Benth) on the stress-induced ulcer

EE
Ebere Ifejirika Ezeonyi Corresponding Chukwuemeka Odumegwu Ojukwu University, Faculty of PharmaceuticalSciences, Department of Pharmacognosy and Traditional Medicinal, Igbariam, Anambra State, Nigeria eberechy92@gmail.com Nigeria
EE
Earnest Oghenesuvwe Erhirhie Chukwuemeka Odumegwu Ojukwu University, Faculty of Pharmaceutical Sciences, Department of Pharmacology and Toxicology, Igbariam, Anambra State, Nigeria Turkey
FO
Felix Ahamefule Onyegbule Nnamdi Azikiwe University, Faculty of Pharmaceutical Sciences, Department of Pharmaceutical and MedicinalChemistry, Agulu, Anambra State, Nigeria Turkey
Pages47-55 PublishedApr 22, 2024 LicenseOpen Access
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IJPBP 1 VOL 4 · 1
VOL 4 · NO 1 · 2024 View issue

Abstract

Lecaniodiscus cupanioides Planch (ex. Benth) is effective in treating burns, fevers, and inflammatory conditions. This study investigates the toxicity effects of the aqueous leaf extract (ALE) of L. cupanioides and its effect on stress-induced ulcers in animal models. The plant was collected and a 1:30 (g/ml) plant powder/solvent ratio was extracted using an ultrasonic bath at 50 °C for 45 min. Up-and-down procedure was used for acute toxicity. During subacute toxicity testing, a total of 20 mice were divided into four groups of five animals each. While group 1 served as control, groups 2, 3, and 4 received 250, 500, and 1000 mg/kg of the extract daily for 21 days. On day 22 nd , animals were sacrificed and samples were collected for hematology, biochemical, and histological analyses. In the stress-induced ulcer activity, male albino mice were randomly separated into 5 groups of 5 animals, treated with the test drug, and then dissected after being stressed using the water immersion model. LD 50 was > 5000 mg/kg, and in biochemical examination, there was a significant decrease in the ALP level at medium and high doses ( p value < 0.05) and non-significant alterations in the values of urea, creatinine, alanine aminotransferase (ALT), aspartate aminotransferase (AST), body weight and hematology parameters ( p > 0.05). The histology results showed no evidence of liver and kidney toxicity. In conclusion, aqueous leaf extract of L. cupanioides was safe following a single dose (at 5000 mg/kg) and following repeated doses for 21 days (at 250, 500, and 1000 mg/kg). This study demonstrated that the extract had a significant effect on stress-induced ulcers at various dose levels when compared to the control (native, negative control, and omeprazole). The animals treated with 200mg/kg and 400mg/kg of the extract showed a significant increase in ulcer score, ulcer index, ulcer severity, and total acidity ( p < 0.05). The ulcer protection ability of the aqueous plant extract was dose-dependent.

Keywords

#Lecaniodiscus cupanioides #Toxicity studies #Stress induced ulcer #Water immersion

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Copyright (c) 2024 Ebere Ifejirika Ezeonyi, Earnest Oghenesuvwe Erhirhie, Felix Ahamefule Onyegbul

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