Determination of cytotoxic, apoptotic, necrotic, antimicrobial and antioxidant activities of Aloe vera and Abies cilicia subsp. cilicica

Abstract

Cancer is one of the most common pathologies in the world, leading to a reduced standard of living and even death for centuries. Despite promising developments in treatment methods in recent years, the expected level of treatment and success hasn’t yet been achieved due to the side effects and cost of treatment methods and the fact that some drugs are still in the trial phase. This situation has encouraged the scientific community to search for natural agents with lower costs and limited side effects. Abies cilicica , also known as fir, and Aloe vera has been used in both food and traditional medicine from the past to the present. In the literature review, it was found that both A. vera and A. cilicica have many beneficial effects, especially anti-inflammatory, antifungal, and wound-healing properties. This study aimed to investigate the antimicrobial, antioxidant, cytotoxic, and apoptotic/necrotic effects of extracts of A. vera from Asphodelaceae and A. cilicica (Ant. Et Kotschy.) subsp. cilicica Carr. from Pinaceae. The best antimicrobial activity was observed against Escerichia coli with a zone diameter of 20.00 ± 3.59 mm and Klebsiella pneumoniae with a zone diameter of 21 ± 5.35 mm. 2KA showed the best effect on antioxidant activity. 2MA + 2KSA showed significant cytotoxic activity on MDA-MB-231 cancer cells. IC 50 values of 1EA + 1KA extract (whole A. cilicica and A. vera dissolved in ethanol) against MDA-MB-231 cell line (IC 50 458.29 ± 19.01 µg/ml) and MCF-7 cell line (IC 50 596.03 ± 5.56 µg/ml) were determined. According to the data obtained from the study, A. vera and A. cilicica were found to have antimicrobial, antioxidant, and cytotoxic effects both alone and synergistically. It is predicted that they can be used especially in cancer treatment.

Keywords

References

1. Abubakar, A. M., Dibal, N. I., Attah, M. O. O., & Chiroma, S. M. (2022). Exploring the antioxidant effects of Aloe vera: Potential role in controlling liver function and lipid profile in high fat and fructose diet (HFFD) fed mice. Pharmacological Research-Modern Chinese Medicine, 4, 100150.
2. Al-Ghazali, L. (2022). The Anti-Candida and the Cytotoxic Activity of Aloe vera Gel Extracts. Journal of Scientific Research in Medical and Biological Sciences, 3(4), 1-9.
3. Arikan, S. (2007). Current status of antifungal susceptibility testing methods. Medical Mycology, 45(7), 569-587.
4. Arsène, M. M. J., Viktorovna, P. I., Alla, M., Mariya, M., Nikolaevitch, S. A., Davares, A. K. L., Yurievna, M. E., Rehailia, M., Gabin, A. A., et al. (2023). Antifungal activity of silver nanoparticles prepared using Aloe vera extract against Candida albicans. Veterinary World, 16(1), 18-26.
5. Avila, H., Rivero, J., Herrera, F., & Fraile, G. (1997). Cytotoxicity of a low molecular weight fraction from Aloe vera (Aloe barbadensis Miller) gel. Toxicon, 35(9), 1423-1430.
6. Awad, L., Fady, B., Khater, C., Roig, A., & Cheddadi, R. (2014). Genetic structure and diversity of the endangered fir tree of Lebanon (Abies cilicica Carr.): Implications for conservation. PLoS One, 9(2), e90086.
7. Baran, H., Güler, P., & Türk, M. (2017). Russula delica FR.’nin sitotoksisite, apoptotik ve nekrotik etkileri. Life Sciences, 12(4), 48-55.
8. Bozkuş, F. (1986). Toros göknarı (Abies ciliciea Carr.)'nın Türkiye'deki doğal yayılış ve silvikültürel özellikleri (The natural distribution and silvicultural characteristics of Abies ciliciea Carr. in Turkey). Journal of the Faculty of Forestry Istanbul University, 0(0).
9. Dalkılıç, S., Korkmaz, İ., Dalkılıç, L. K., Akay, G., & Fidan, S. (2022). In vitro cytotoxic effects of Smilax aspera L. roots on cancer cell lines. Food Bioscience, 46, 101501.
10. Dayisoylu, K. S., Duman, A. D., Alma, M. H., & Digrak, M. (2009). Antimicrobial activity of the essential oils of rosin from cones of Abies cilicica subsp. cilicica. African Journal of Biotechnology, 8(19), 5021-5024.
11. Dığrak, M., İlçim, A., & Hakkı Alma, M. (1999). Antimicrobial activities of several parts of Pinus brutia, Juniperus oxycedrus, Abies cilicia, Cedrus libani and Pinus nigra. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 13(7), 584-587.
12. Erdogan, M. K., Agca, C. A., & Gecibesler, İ. H. (2020). The antiproliferative potential of isolated emodin and aloe-emodin from Rheum ribes on different cancer cell lines. Biyolojik Çeşitlilik ve Koruma, 13(2), 160-168.
13. Eryilmaz, M., Tosun, A., & Tümen, İ. (2016). Antimicrobial activity of some species from Pinaceae and Cupressaceae. Turkish Journal of Pharmaceutical Sciences, 13(1), 35-40.
14. Fenig, E., Nordenberg, J., Beery, E., Sulkes, J., & Wasserman, L. (2004). Combined effect of aloe-emodin and chemotherapeutic agents on the proliferation of an adherent variant cell line of Merkel cell carcinoma. Oncology Reports, 11(1), 213-217.
15. Fujita, T., Sezik, E., Tabata, M., Yesilada, E., Honda, G., Takeda, Y., Tanaka, T., & Takaishi, Y. (1995). Türkiye’de Geleneksel Tababet VII. Orta ve Bati Karadeniz Bölgelerinde Halk Haçlari. Economic Botany, 49, 406-422.
16. Guo, X., & Mei, N. (2016). Aloe vera: A review of toxicity and adverse clinical effects. Journal of Environmental Science and Health, Part C, 34(2), 77-96.
17. Guzeldag, G., Kadioglu, L., Mercimek, A., & Matyar, F. (2014). Peliminary examination of herbal extracts on the inhibition of Helicobacter pylori. African Journal of Traditional, Complementary and Alternative Medicines, 11(1), 93-96.
18. Hamman, J. (2008). Composition and applications of Ocimum gratissimum leaf. Molecules, 13(8), 1599-1616.
19. Haque, S., Saha, S., Salma, U., Nishi, M., & Rahaman, M. (2019). Antibacterial Effect of Aloe vera (Aloe barbadensis) leaf gel against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae. Mymensingh Medical Journal: MMJ, 28(3), 490-496.
20. Karakaş, D. (2013). Kültür ortamında fibroblastların varlığında A-549 akciğer kanseri hücre hattının farklı kemoterapi ilaçlarına verdiği yanıtın araştırılması. (Master Thesis). Uludağ Üniversitesi.
21. Kizil, M., Kizil, G., Yavuz, M., & Aytekin, Ç. (2002). Antimicrobial activity of resins obtained from the roots and stems of Cedrus libani and Abies cilicia. Applied Biochemistry and Microbiology, 38, 144-146.
22. Kuo, P. L., Lin, T. C., & Lin, C. C. (2002). The antiproliferative activity of aloe-emodin is through p53-dependent and p21-dependent apoptotic pathway in human hepatoma cell lines. Life Sciences, 71(16), 1879-1892.
23. López-Tirado, J., Moreno-García, M., Romera-Romera, D., Zarco, V., & Hidalgo, P. J. (2023). Forecasting the circum-Mediterranean firs (Abies spp., Pinaceae) distribution: an assessment of a threatened conifers’ group facing climate change in the twenty-first century. New Forests, 2023, 1-14.
24. Majumder, R., Das, C. K., & Mandal, M. (2019). Lead bioactive compounds of Aloe vera as potential anticancer agent. Pharmacological Research, 148, 104416.
25. Majumder, R., Parida, P., Paul, S., & Basak, P. (2020). In vitro and in silico study of Aloe vera leaf extract against human breast cancer. Natural Product Research, 34(16), 2363-2366.
26. Manirakiza, A., Irakoze, L., & Manirakiza, S. (2021). Aloe and its effects on cancer: a narrative literature review. The East African Health Research Journal, 5(1), 1-16.
27. Nejatzadeh-Barandozi, F. (2013). Antibacterial activities and antioxidant capacity of Aloe vera. Organic and Medicinal Chemistry Letters, 3, 1-8.
28. Pichette, A., Larouche, P. L., Lebrun, M., & Legault, J. (2006). Composition and antibacterial activity of Abies balsamea essential oil. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 20(5), 371-373.
29. Stanley, M. C., Ifeanyi, O. E., & Eziokwu, O. G. (2014). Antimicrobial effects of Aloe vera on some human pathogens. International Journal of Current Microbiology and Applied Sciences, 3(3), 1022-1028.
30. Tong, X., Li, M., Li, D., Lao, C., Chen, J., Xu, W., Du, J., Zhang, M., Yang, X., et al. (2021). Aloe vera gel extract: Safety evaluation for acute and chronic oral administration in Sprague-Dawley rats and anticancer activity in breast and lung cancer cells. Journal of Ethnopharmacology, 280, 114434.
31. Tumen, I., Akkol, E. K., Süntar, I., & Keleş, H. (2011). Wound repair and anti-inflammatory potential of essential oils from cones of Pinaceae: preclinical experimental research in animal models. Journal of Ethnopharmacology, 137(3), 1215-1220.
32. Verbanac, D., Malik, R., Chand, M., Kushwaha, K., Vashist, M., Matijašić, M., Stepanić, V., Perić, M., Paljetak, H. Č., et al. (2016). Synthesis and evaluation of antibacterial and antioxidant activity of novel 2-phenyl-quinoline analogs derivatized at position 4 with aromatically substituted 4 H-1, 2, 4-triazoles. Journal of Enzyme Inhibition and Medicinal Chemistry, 31(sup2), 104-110.
33. Vishnoi, S. P., Ghosh, A. K., Debnath, B., Samanta, S., Gayen, S., & Jha, T. (2007). Antibacterial activity of Abies webbiana. Fitoterapia, 78(2), 153-155.
34. Wasserman, L., Avigad, S., Beery, E., Nordenberg, J., & Fenig, E. (2002). The effect of aloe emodin on the proliferation of a new merkel carcinoma cell line. The American Journal of Dermatopathology, 24(1), 17-22.
35. Yaglioglu, A. S., Yaglioglu, M. S., Tosyalıoglu, N., Adem, S., & Demirtas, I. (2022). Chemical profiling, in vitro biological activities and Pearson correlation between chemical profiling and anticancer activities of four Abies species from Turkey. South African Journal of Botany, 151, 600-613.
36. Yener, D. Y. (2012). Abies Taxa of Turkey and Their Visual Characteristics. Kastamonu University Journal of Forestry Faculty, 12(3), 259-262.
37. Zeleke, D., Eswaramoorthy, R., Belay, Z., & Melaku, Y. (2020). Synthesis and antibacterial, antioxidant, and molecular docking analysis of some novel quinoline derivatives. Journal of Chemistry, 2020, 1-16.

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Copyright (c) 2023 Semih Dalkılıç, Lütfiye Kadıoğlu Dalkılıç, Mert Can Küçüktüfekçi, Dilek Arslan Ateşşahin, Ayşenur Çelik, Özgecan Gülaçar, Ayşenur Çil